29 lines
1.7 KiB
Markdown
29 lines
1.7 KiB
Markdown
---
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title: "GC-content"
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chunk: 2/2
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source: "https://en.wikipedia.org/wiki/GC-content"
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category: "reference"
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tags: "science, encyclopedia"
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date_saved: "2026-05-05T09:07:14.065445+00:00"
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instance: "kb-cron"
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---
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=== Molecular biology ===
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In polymerase chain reaction (PCR) experiments, the GC-content of short oligonucleotides known as primers is often used to predict their annealing temperature to the template DNA. A higher GC-content level indicates a relatively higher melting temperature.
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Many sequencing technologies, such as Illumina sequencing, have trouble reading high-GC-content sequences. Bird genomes are known to have many such parts, causing the problem of "missing genes" expected to be present from evolution and phenotype but never sequenced — until improved methods were used.
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=== Systematics ===
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The species problem in non-eukaryotic taxonomy has led to various suggestions in classifying bacteria, and the ad hoc committee on reconciliation of approaches to bacterial systematics of 1987 has recommended use of GC-ratios in higher-level hierarchical classification. For example, the Actinomycetota are characterised as "high GC-content bacteria". In Streptomyces coelicolor A3(2), GC-content is 72%. With the use of more reliable, modern methods of molecular systematics, the GC-content definition of Actinomycetota has been abolished and low-GC bacteria of this clade have been found.
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== Software tools ==
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GCSpeciesSorter and TopSort are software tools for classifying species based on their GC-contents.
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== See also ==
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Codon usage bias
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== References ==
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== External links ==
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Table with GC-content of all sequenced prokaryotes
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Taxonomic browser of bacteria based on GC ratio on NCBI website.
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GC ratio in diverse species. |