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=== Adaptation to hydrostatic pressure === Deep-sea fish have different adaptations in their proteins, anatomical structures, and metabolic systems to survive in the Deep sea, where the inhabitants have to withstand great amount of hydrostatic pressure. While other factors like food availability and predator avoidance are important, the deep-sea organisms must have the ability to maintain well-regulated metabolic system in the face of high pressures. In order to adjust for the extreme environment, these organisms have developed unique characteristics. Proteins are affected greatly by the elevated hydrostatic pressure, as they undergo changes in water organization during hydration and dehydration reactions of the binding events. This is due to the fact that most enzyme-ligand interactions form through charged or polar non-charge interactions. Because hydrostatic pressure affects both protein folding and assembly and enzymatic activity, the deep sea species must undergo physiological and structural adaptations to preserve protein functionality against pressure. Actin is a protein that is essential for different cellular functions. The α-actin serves as a main component for muscle fiber, and it is highly conserved across numerous different species. Some Deep-sea fish developed pressure tolerance through the change in mechanism of their α-actin. In some species that live in depths greater than 5 km (3.1 mi), C.armatus and C.yaquinae have specific substitutions on the active sites of α-Actin, which serves as the main component of muscle fiber. These specific substitutions, Q137K and V54A from C.armatus or I67P from C.yaquinae are predicted to have importance in pressure tolerance. Substitution in the active sites of actin result in significant changes in the salt bridge patterns of the protein, which allows for better stabilization in ATP binding and sub unit arrangement, confirmed by the free energy analysis and molecular dynamics simulation. It was found that deep sea fish have more salt bridges in their actins compared to fish inhabiting the upper zones of the sea. In relations to protein substitution, specific osmolytes were found to be abundant in deep sea fish under high hydrostatic pressure. For certain chondrichthyans, it was found that Trimethylamine N-oxide (TMAO) increased with depth, replacing other osmolytes and urea. Due to the ability of TMAO being able to protect proteins from high hydrostatic pressure destabilizing proteins, the osmolyte adjustment serves are an important adaptation for deep sea fish to withstand high hydrostatic pressure. Deep-sea organisms possess molecular adaptations to survive and thrive in the deep oceans. Mariana hadal snailfish developed modification in the Osteocalcin(burlap) gene, where premature termination of the gene was found. Osteocalcin gene regulates bone development and tissue mineralization, and the frameshift mutation seems to have resulted in the open skull and cartilage-based bone formation. Due to high hydrostatic pressure in the deep sea, closed skulls that organisms living on the surface develop cannot withstand the enforcing stress. Similarly, common bone developments seen in surface vertebrates cannot maintain their structural integrity under constant high pressure.

== Exploration ==

It has been suggested that more is known about the Moon than the deepest parts of the ocean. This is a common misconception based on a 1953 statement by George E.R. Deacon published in the Journal of Navigation, and largely refers to the scarce amount of seafloor bathymetry available at the time. The similar idea that more people have stood on the moon than have been to the deepest part of the ocean is likewise dubious. Still, the deep-sea remains one of the least explored regions on planet Earth. Pressures even in the mesopelagic become too great for traditional exploration methods, demanding alternative approaches for deep-sea research. Baited camera stations, small crewed submersibles, and ROVs (remotely operated vehicles) are three methods utilized to explore the ocean's depths. Because of the difficulty and cost of exploring this zone, current knowledge is limited. Pressure increases at approximately one atmosphere for every 10 meters meaning that some areas of the deep sea can reach pressures of above 1,000 atmospheres. This not only makes great depths very difficult to reach without mechanical aids, but also provides a significant difficulty when attempting to study any organisms that may live in these areas as their cell chemistry will be adapted to such vast pressures.

== See also == Deep ocean water Cold, salty water deep below the surface of Earth's oceans Submarine landslide Landslides that transport sediment across the continental shelf and into the deep ocean The Blue Planet 2001 British nature documentary television series Blue Planet II 2017 British nature documentary television series Nepheloid layer Layer of water in deep sea Biogenous ooze Oceans portal

== References ==

== External links ==

Deep Sea Foraminifera Deep Sea Foraminifera from 4400 meters depth, Antarctica an image gallery and description of hundreds of specimens Deep Ocean Exploration on the Smithsonian Ocean Portal Deep-Sea Creatures Facts and images from the deepest parts of the ocean How Deep Is The Ocean Archived 2016-06-15 at the Wayback Machine Facts and infographic on ocean depth