2.8 KiB
| title | chunk | source | category | tags | date_saved | instance |
|---|---|---|---|---|---|---|
| Interferometry | 10/10 | https://en.wikipedia.org/wiki/Interferometry | reference | science, encyclopedia | 2026-05-05T09:43:15.174742+00:00 | kb-cron |
Phase contrast and differential interference contrast (DIC) microscopy are important tools in biology and medicine. Most animal cells and single-celled organisms have very little color, and their intracellular organelles are almost totally invisible under simple bright field illumination. These structures can be made visible by staining the specimens, but staining procedures are time-consuming and kill the cells. As seen in Figs. 24 and 25, phase contrast and DIC microscopes allow unstained, living cells to be studied. DIC also has non-biological applications, for example in the analysis of planar silicon semiconductor processing. Angle-resolved low-coherence interferometry (a/LCI) uses scattered light to measure the sizes of subcellular objects, including cell nuclei. This allows interferometry depth measurements to be combined with density measurements. Various correlations have been found between the state of tissue health and the measurements of subcellular objects. For example, it has been found that as tissue changes from normal to cancerous, the average cell nuclei size increases. Phase-contrast X-ray imaging (Fig. 26) refers to a variety of techniques that use phase information of a coherent x-ray beam to image soft tissues. (For an elementary discussion, see Phase-contrast x-ray imaging (introduction). For a more in-depth review, see Phase-contrast X-ray imaging.) It has become an important method for visualizing cellular and histological structures in a wide range of biological and medical studies. There are several technologies being used for x-ray phase-contrast imaging, all utilizing different principles to convert phase variations in the x-rays emerging from an object into intensity variations. These include propagation-based phase contrast, Talbot interferometry, Moiré-based far-field interferometry, refraction-enhanced imaging, and x-ray interferometry. These methods provide higher contrast compared to normal absorption-contrast x-ray imaging, making it possible to see smaller details. A disadvantage is that these methods require more sophisticated equipment, such as synchrotron or microfocus x-ray sources, x-ray optics, or high resolution x-ray detectors.
== See also == Coherence Coherence scanning interferometry Fine Guidance Sensor (HST) (HST FGS are interferometers) Holography Interferometric visibility Interference lithography List of types of interferometers Ramsey interferometry Seismic interferometry Superposition principle Very-long-baseline interferometry Zero spacing flux
== References ==
Media related to Interferometry at Wikimedia Commons