---
title: "History of botany"
chunk: 9/10
source: "https://en.wikipedia.org/wiki/History_of_botany"
category: "reference"
tags: "science, encyclopedia"
date_saved: "2026-05-05T03:59:20.683361+00:00"
instance: "kb-cron"
---

Until the 1860s, it was believed that species had remained unchanged through time: each biological form was the result of an independent act of creation and therefore absolutely distinct and immutable. But the hard reality of geological formations and strange fossils needed scientific explanation. Charles Darwin's Origin of Species (1859) replaced the assumption of constancy with the theory of descent with modification. Phylogeny became a new principle as "natural" classifications became classifications reflecting, not just similarities, but evolutionary relationships. Wilhelm Hofmeister established that there was a similar pattern of organisation in all plants expressed through the alternation of generations and extensive homology of structures.
German writer Johann Wolfgang von Goethe (1749–1832), a polymath, had interests and influence that extended into botany. In Die Metamorphose der Pflanzen (1790), he provided a theory of plant morphology (he coined the word "morphology") and he included within his concept of "metamorphosis" modification during evolution, thus linking comparative morphology with phylogeny. Though the botanical basis of his work has been challenged, there is no doubt that he prompted discussion and research on the origin and function of floral parts. His theory probably stimulated the opposing views of German botanists Alexander Braun (1805–1877) and Matthias Schleiden who applied the experimental method to the principles of growth and form that were later extended by Augustin de Candolle (1778–1841).

=== Carbon fixation (photosynthesis) ===

At the start of the 19th century, the idea that plants could synthesize almost all their tissues from atmospheric gases had not yet emerged. The energy component of photosynthesis, the capture and storage of the Sun's radiant energy in carbon bonds (a process on which all life depends) was first elucidated in 1847 by Mayer, but the details of how this was done would take many more years. Chlorophyll was named in 1818 and its chemistry gradually determined, to be finally resolved in the early 20th century. The mechanism of photosynthesis remained a mystery until the mid-19th century when Sachs, in 1862, noted that starch was formed in green cells only in the presence of light, and in 1882, he confirmed carbohydrates as the starting point for all other organic compounds in plants. The connection between the pigment chlorophyll and starch production was finally made in 1864 but tracing the precise biochemical pathway of starch formation did not begin until about 1915.

=== Nitrogen fixation ===

Significant discoveries relating to nitrogen assimilation and metabolism, including ammonification, nitrification and nitrogen fixation (the uptake of atmospheric nitrogen by symbiotic soil microorganisms) had to wait for advances in chemistry and bacteriology in the late 19th century and this was followed in the early 20th century by the elucidation of protein and amino-acid synthesis and their role in plant metabolism. With this knowledge, it was then possible to outline the global nitrogen cycle.

== Twentieth century ==

20th century science grew out of the solid foundations laid by the breadth of vision and detailed experimental observations of the 19th century. A vastly increased research force was now rapidly extending the horizons of botanical knowledge at all levels of plant organisation from molecules to global plant ecology. There was now an awareness of the unity of biological structure and function at the cellular and biochemical levels of organisation. Botanical advance was closely associated with advances in physics and chemistry with the greatest advances in the 20th century mainly relating to the penetration of molecular organisation. However, at the level of plant communities it would take until mid century to consolidate work on ecology and population genetics.
By 1910, experiments using labelled isotopes were being used to elucidate plant biochemical pathways, to open the line of research leading to gene technology. On a more practical level, research funding was now becoming available from agriculture and industry.

=== Molecules ===

In 1903, chlorophylls a and b were separated by thin layer chromatography then, through the 1920s and 1930s, biochemists, notably Hans Krebs (1900–1981) and Carl (1896–1984) and Gerty Cori (1896–1957) began tracing out the central metabolic pathways of life. Between the 1930s and 1950s, it was determined that ATP, located in mitochondria, was the source of cellular chemical energy and the constituent reactions of photosynthesis were progressively revealed. Then, in 1944, DNA was extracted for the first time. Along with these revelations, there was the discovery of plant hormones or "growth substances", notably auxins, (1934) gibberellins (1934) and cytokinins (1964) and the effects of photoperiodism, the control of plant processes, especially flowering, by the relative lengths of day and night.
Following the establishment of Mendel's laws, the gene-chromosome theory of heredity was confirmed by the work of August Weismann who identified chromosomes as the hereditary material. Also, in observing the halving of the chromosome number in germ cells he anticipated work to follow on the details of meiosis, the complex process of redistribution of hereditary material that occurs in the germ cells. In the 1920s and 1930s, population genetics combined the theory of evolution with Mendelian genetics to produce the modern synthesis. By the mid-1960s, the molecular basis of metabolism and reproduction was firmly established through the new discipline of molecular biology. Genetic engineering, the insertion of genes into a host cell for cloning, began in the 1970s with the invention of recombinant DNA techniques and its commercial applications applied to agricultural crops followed in the 1990s. There was now the potential to identify organisms by molecular "fingerprinting" and to estimate the times in the past when critical evolutionary changes had occurred through the use of "molecular clocks".

=== Computers, electron microscopes and evolution ===